HILIC Columns and Accessories - YMC-Triart Diol-HILIC
High-Performance Columns for Polar Compound Separations
Hydrophilic Interaction Liquid Chromatography (HILIC) is a powerful separation mode specifically designed for polar organic compounds that show poor retention in reversed-phase systems.
In conventional reversed-phase HPLC, highly polar compounds often elute near the solvent front or co-elute with matrix components, resulting in poor resolution and ion suppression during MS detection.
HILIC solves this challenge by using a polar stationary phase and a mobile phase rich in organic solvent (typically acetonitrile). A thin, water-enriched layer forms on the silica surface, promoting partitioning and hydrogen-bond interactions that enhance the retention and selectivity of polar analytes.
YMC’s HILIC columns provide exceptional reproducibility, mechanical stability, and compatibility with LC-MS, UHPLC, and high-throughput workflows, which make them ideal for pharmaceuticals, metabolites, and bioanalytical compounds.
Characteristics
- Superior chemical durability
- Ideal for the separation of polar and hydrophilic compounds that are not retained by reversed phase chromatography
- Can be used for LC/MS
Applications
Typical applications include: amino acids, water soluble vitamins, sugars, antibodies, proteins, peptides
Specifications
| Stationary Phase | dihydroxypropyl (USP L20) |
|---|---|
| Bonding | Trifunctional |
| Endcapping | None |
| Mode | HILIC |
| Particle Size | 1.9, 3, 5 µm |
| Pore Size | 120 Å |
| Carbon Content | 12% |
| pH Range | 2 – 10 |
| Temperature Upper Limit | 50 °C |
| Temperature Recommended Range | 20 – 40 °C |
Available Formats
UHPLC Resin (1.9 µm)
- Prepacked columns & guards
- Capillary (< 1 mm ID)
- Analytical (1.0 – 8.0 mm ID)
- Biocompatible options (2.1 & 4.6 mm ID)
Analytical Resin (3 – 5 µm)
- Prepacked columns & guards
- Capillary (< 1 mm ID)
- Analytical (1.0 – 8.0 mm ID)
- Biocompatible & high-pressure options (2 – 4.6 mm ID)
- Preparative (10 mm ID & above)
- Bulk resin
LC Columns & Accessories
| Product | Phase / Type | Separation Mode(s) | Pore Sizes | Particle Sizes | pH Range | Available Formats | Link to Order |
|---|---|---|---|---|---|---|---|
| YMC-Triart Diol-HILIC | Dihydroxypropyl | HILIC | 120 Å | 3 µm5 µm1.9 µm | 2 - 10 | UHPLCAnalytical (1 - 8 mm ID)Preparative (10 mm ID & above)Capillary (< 1 mm ID)Biocompatible |
When to Use HILIC Chromatography
HILIC is ideal when:
- You need to retain and resolve highly polar compounds not amenable to reversed-phase chromatography
- Polar analytes co-elute near the void in RP-HPLC or suffer from ion suppression
- Your application involves polar pharmaceuticals, metabolites, amino acids, carbohydrates, or nucleosides
- You require MS-compatible separations with high organic content for improved ionization efficiency
Why Choose YMC HILIC Columns?
Superior Retention of Polar Compounds
YMC HILIC columns create a stable, water-enriched stationary-phase layer that enhances interaction with polar analytes, delivering strong, predictable retention and sharp peak shapes.
Wide Compatibility and Robustness
Designed for both HPLC and UHPLC systems, YMC HILIC phases offer excellent reproducibility, mechanical strength, and low bleed for LC-MS and high-throughput applications.
Enhanced Selectivity and Versatility
Multiple bonded-phase chemistries (neutral, zwitterionic, and diol-based) enable fine control of hydrophilic selectivity for diverse compound classes.
Reproducible Results and Scale-Up Support
Manufactured from high-purity silica and controlled bonding processes, YMC ensures batch-to-batch consistency and scalability from analytical to preparative workflows.
MS-Friendly Performance
High organic content in HILIC mobile phases improves ionization efficiency and sensitivity for LC-MS applications—ideal for metabolomics and bioanalytical assays.
HILIC Chromatography FAQs
Q: What is the main advantage of HILIC over reversed-phase chromatography?
HILIC provides superior retention for highly polar compounds that elute too early or not at all in reversed-phase systems.
Q: What mobile phase composition is typically used in HILIC?
Common mobile phases contain 60–95% acetonitrile with a small proportion of aqueous buffer to establish the water-rich stationary-phase layer needed for polar retention.
Q: Are YMC HILIC columns compatible with LC-MS?
Yes. YMC HILIC columns are fully MS-compatible, offering low bleed, high reproducibility, and enhanced ionization efficiency.
Q: What particle sizes and formats are available?
YMC HILIC columns are available in sub-2 µm (UHPLC) and 3–5 µm (HPLC) particle sizes for analytical and high-throughput workflows. Preparative formats are available upon request.
Need Help Choosing the Right HILIC Column?
Our chromatography specialists can help you identify the optimal stationary phase and operating conditions for your specific polar analytes.
YMC also offers custom column packing, method development kits, and scale-up support to ensure success from discovery to process development.
Contact us today or browse our selection of columns.
