YMC-Pack C4
Classical phase with low hydrophobicity.
YMC-Pack C4 columns are less hydrophobic than C8 or C18 materials, and generally utilize more aqueous eluents. When compared to C8 and C18 columns (using the same eluents), C4 stationary phases show significantly shorter retention for non-polar compounds. Retention of polar compounds, however, is not significantly affected. Therefore, YMC-Pack C4 is ideal for analyzing mixtures with a wide range of component polarity. This is due to the C4 bonded phase exhibiting shorter analysis time while still maintaining high resolution when compared to longer-chain bonded chemistries.
YMC-Pack C4 (Butyl) columns are available with three pore sizes to accommodate optimum separations of virtually any molecular-weight compound. The 120 Å phase is used extensively with non-polar peptides which are too strongly retained on C8 and C18. The 200 Å and 300 Å phase columns effectively resolve many classes of proteins and biopolymers. The wide pore butyl chemistry allows minimal distortion of tertiary conformation for large biomolecules and results in samples that are pure, concentrated, and high in biological activity.
Characteristics
- Ideally suited for biological separations
- High-coverage monomeric bonding on perfectly spherical silica supports
- Fully endcapped
Applications
Typical applications include: biological separations, polar compounds
Analytical Specifications
| YMC-Pack C4 | |
|---|---|
| Stationary Phase | C4 (USP L26) |
| Bonding | Monomeric |
| Endcapping | Yes |
| Mode | Reversed Phase |
| Particle Size | 3, 5 µm |
| Pore Size | 120, 200, 300 Å |
| Carbon Content | 7%, 5%, 3% |
| pH Range | 2 – 7.5 |
| Temperature Upper Limit | 50 °C |
| Temperature Recommended Range | 20 – 40 °C |
*not all combinations of particle & pore size are available
Preparative Specifications
| YMC-Pack C4-HG | |
|---|---|
| Stationary Phase | C4 (USP L26) |
| Bonding | Monomeric |
| Endcapping | Yes |
| Mode | Reversed Phase |
| Particle Size | 10, 15, 20, 50 µm |
| Pore Size | 120, 200, 300 Å |
| Carbon Content | 7%, 5%, 3% |
| pH Range | 2 – 7.5 |
*not all combinations of particle & pore size are available
YMC-Pack C4 with particle sizes of 10 µm and higher are available as part of the YMC*Gel High Grade (HG) series. These resins boast improved mechanical stability as well as narrower particle size and pore size distribution.
YMC-Pack C4-HG can be purchased in prepacked analytical and preparative columns or in bulk resin format.
Available Formats
- Prepacked columns & guards
- Capillary (< 1 mm ID)
- Analytical (1.0 – 8.0 mm ID)
- Preparative (10 mm ID & above)
- Bulk resin
LC Columns & Accessories
| Product | Phase / Type | Separation Mode(s) | Pore Sizes | Particle Sizes | pH Range | Available Formats | Link to Order |
|---|---|---|---|---|---|---|---|
| YMC-Pack C4 | C4 | Reversed Phase | 120 Å200 Å300 Å | 3 µm5 µm10 µm15 µm20 µm50 µm | 2 - 7.5 | Analytical (1 - 8 mm ID)Preparative (10 mm ID & above)Capillary (< 1 mm ID) |
