YMC-BioPro IEX
Ion exchange columns for separation of proteins, peptides, and nucleic acids.
YMC-BioPro IEX is a family of ion exchange (IEX) columns and bulk resins for analytical separations to benchtop and process/pilot-scale purifications of proteins, peptides, oligonucleotides, and other biomolecules.
BioPro IEX is based on a hydrophilic polymethacrylate bead which exhibits very low non-specific adsorption and may therefore reduce the need for organic solvents in the mobile phase.
These columns exhibit consistent retention, separation, and recovery, and are ideal for method validation due to proven lot-to-lot reproducibility.
Column Types
YMC-BioPro IEX columns are available with porous and non-porous particles for strong anion exchange (SAX) and strong cation exchange (SCX).
Columns packed with the porous materials (QA & SP) are advantageous for high-load analytical separations and laboratory-scale purification as they maintain strong peak shape even with increased load.
The non-porous formats (QF & SF) are highly efficient, and are therefore ideal for high-throughput analytical separations requiring high resolution.
| Product Name | BioPro IEX QA | BioPro IEX QF | |
|---|---|---|---|
| Charged Group | -CH2N+(CH3)3 | -CH2N+(CH3)3 | |
| Porosity | Porous | Non-porous | |
| pH Range | 2-12 | 2-12 | |
| Particle Size | 5µm | 3µm, 5µm | 6µm |
| Available Column Formats | PEEK Hardware (Analytical) | PEEK Hardware (Analytical) | Stainless Steel Hardware (Prep) |
Bulk Resin
One of the advantages of YMC’s BioPro IEX resins is that they are fully scalable, exhibiting consistent chromatographic behavior across all particle sizes. Preparative grade resins with 10 – 75 μm particles are available in mL and L quantities.
YMC-BioPro IEX SmartSep Q/S (10, 20, or 30 μm), which show high resolution and recovery even at a high flow rate and high loading condition, are suitable for an intermediate purification step and a polishing step of mAbs, proteins, peptides, and oligonucleotides. YMC-BioPro IEX Q/S (75 μm) are designed for capture and intermediate purification of biopharmaceuticals.
Screening Kits & MiniChrom® Columns
YMC-BioPro IEX Screening Kits contain five cartridges, each packed with 1mL or 5mL of 20μm, 30μm, or 75μm BioPro resin. These kits are ideal for media screening, development of purification methods, and loadability studies.
MiniChrom® BioPro Columns are polypropylene LC columns packed with YMC-BioPro IEX stationary phase material. These columns are designed for operating conditions up to 30 °C and 30 bar (435 psi). They are useful for bench-scale resin cleaning and method development, sample cleaning, sample prep/cleanup, bind-and-recover sample purification, and flow-through purification.
LC Columns & Accessories
| Product | Phase / Type | Separation Mode(s) | Pore Sizes | Particle Sizes | pH Range | Available Formats | Link to Order |
|---|---|---|---|---|---|---|---|
| YMC-BioPro IEX | IEX | Ion Exchange (IEX) | Non-Porous1000 Å | 3 µm5 µm10 µm20 µm75 µm6 µm30 µm | 2 - 12 | Analytical (1 - 8 mm ID)BiocompatiblePreparative (10 mm ID & above)Screening Kit |
