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Two-Step RP Purification of Tirzepatide

Scaling Up Tirzepatide Purification: From 20% Crude to >99% Purity
With the surge in demand for GLP-1 receptor agonists, efficient and scalable purification strategies for complex lipopeptides like Tirzepatide have never been more critical.

In this latest Application Note by YMC, learn how a two-step Reverse Phase (RP) purification strategy achieves exceptional results:
► Step 1 (Bulk Impurity Removal): YMC-Triart Prep Bio200 C8 boosts initial purity from 20% crude up to 93%.
► Step 2 (Polishing): YMC-Triart Prep C4-S operating at pH 8.0 delivers a final HPLC purity of 99.7%.

The hybrid-silica base of the YMC-Triart Prep series allows for operation at higher pH levels, overcoming a major limitation of traditional silica-based phases and enabling optimal resolution for challenging peptides.

Download the Application Note.

Posted on July 30, 2026.

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