YMC-Pack ODS-AQ
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Classical polar phase.
YMC-Pack ODS-AQ is a unique reversed-phase material with both a hydrophobic high carbon loading and a relatively hydrophilic surface. Because of the hydrophilic surface, ODS-AQ can be “wetted” with polar eluents. It provides chromatographers reproducible retention and excellent peak shape in highly aqueous mobile phases including 100% water. This is due to the C18 ligands on ODS-AQ actually being lifted from the surface by the action of the eluents penetrating the silica pores.
Selectivity on YMC-Pack ODS-AQ can be subtly different from that seen on conventional ODS columns. Differences in selectivity are most often encountered when analyzing mixtures of relatively polar compounds. Typically, longer retention and greater resolution are observed with ODS-AQ. Available in both 120Å and 200Å pore sizes, YMC-Pack ODS-AQ is well-suited for analysis and purification of peptides and other biomolecules.
Characteristics
- Recommended for analysis of highly polar compounds
- Eliminates need for paired ion reagents
- Strong retention in aqueous eluents
- Enhanced separation of biomolecules such as peptides, proteins, and nucleotides
Applications
Typical applications include: strong polar compounds
Specifications
| Analytical Specifications | |
|---|---|
| Stationary Phase | C18 (USP L1) |
| Bonding | Monomeric |
| Endcapping | Yes |
| Mode | Reversed Phase |
| Particle Size* | 3, 5 µm |
| Pore Size* | 120, 200 Å |
| Carbon Content | 14%, 10% |
| pH Range | 2 – 7.5 |
| Temperature Upper Limit | 50 °C |
| Temperature Recommended Range | 20 – 40 °C |
*not all combinations of particle & pore size are available
| Preparative Specifications | |
|---|---|
| Stationary Phase | C18 (USP L1) |
| Bonding | Monomeric |
| Endcapping | Yes |
| Mode | Reversed Phase |
| Particle Size* | 10, 15, 20, 50 µm |
| Pore Size* | 120, 200 Å |
| Carbon Content | 14%, 10% |
| pH Range | 2 – 7.5 |
*not all combinations of particle & pore size are available
Preparative Resin
YMC-Pack ODS-AQ with particle sizes of 10 µm and higher are available as part of the YMC*Gel High Grade (HG) series. These resins boast improved mechanical stability as well as narrower particle size and pore size distribution. YMC-Pack ODS-AQ-HG can be purchased in prepacked analytical and preparative columns or in bulk resin format.
