YMC-Pack CN

YMC-Pack CN can be used for both normal phase and reversed phase analyses due to the medium polarity of the cyanopropyl phase bonded to the silica base particle. It can be used in normal phase with low-polarity mobile phases such as hexane, or in reversed phase with highly-polar mobile phases such as methanol and water. YMC-Pack CN can be useful for shortening analysis time in situations where retention is substantial using C18 or C8, or when separation optimization is difficult.

In reversed phase mode, cyano columns are the most polar and least retentive of all reversed phase packings. Extremely hydrophobic compounds which do not elute in standard C18 and C8 packings can be retained using typical reversed phase eluents. In addition, the unique nitrile chemistry provides different selectivity from both phenyl and standard aliphatic (C18, C8, and C4) reversed phase packings. Large pore size 300Å packing materials are also available for the analysis of biomolecules in order to utilize this unique selectivity.

In normal phase mode, cyano is a bonded phase that can be used as an alternative to silica. Bonded normal phase columns have the advantage of faster equilibration and more uniform surface activity compared to bare silica gel.

*Tip: To extend column lifetime, continued switching back and forth 
between normal and reversed phase solvents should be avoided.