MacroSep IEX Q
Anion Exchange Resin for Purification of Large Biomolecules
Ion exchange chromatography is an ideal technique for purifying biomolecules with high productivity. As the target substances become larger, the demands on process resins are changing. YMC has developed a new and innovative resin to target large biomolecules and particles such as adeno-associated viruses (AAVs), protein complexes, and plasmid DNA (pDNA): MacroSep IEX Q!
This new strong anion exchange resin offers an optimized macro-porous structure that ensures efficient purification of these molecules. With its high binding capacity for large particles and molecules in addition to high separation efficiency even at elevated flow rates, MacroSep IEX Q is a perfect addition to DSP platforms.
Features & Specifications
- Macro-porous structure for the separation of large biomolecules such as AAVs
- Strong anion exchanger providing high dynamic binding capacity (DBC)
- Ideal particle size (30 µm) for efficient purifications at improved resolution
- High resolution even at increased flow rate
| Matrix | methacrylate-based hydrophilic porous polymer |
|---|---|
| Charged Group | -R-N+(CH3)3 |
| Particle Size | 30 µm |
| Pore Size | 900 nm |
| pH Range | 2 – 12 |
| Pressure Limit | 2 MPa (for regular use) |
| 3 MPa (upper limit) | |
| Temperature | 4 – 60° C |
| Ion Exchange Capacity | >0.08 meq/mL-resin |
| Dynamic Binding Capacity | 30 mg/mL-Resin (Thyroglobulin) |
Separation of Large Biomolecules
With MacroSep IEX Q, large biomolecules and viral particles are efficiently purified.
This application shows the separation of full and empty AAV – a challenging separation task with high demands on resolution and recovery.
With MacroSep IEX Q both species are separated efficiently.
Excellent Resolution at Elevated Flow Rates
MacroSep IEX Q provides excellent resolution, even with increased flow rates. The pressure-flow curve reveals a completely linear dependence. In a direct comparison with an alternative resin at two different flow rates, MacroSep IEX Q exhibited superior resolution both times. This characteristic directly leads to improved process conditions and higher productivity.
High-Resolution Separation of Plasmid DNA
During the production and purification of Plasmid DNA, the separation of super-coiled (Sc) pDNA from the non-functional linearized (L) and nicked open-circular (Oc) species poses a major challenge. This is because all three forms are very similar. In this application, MacroSep IEX Q successfully produces a high-resolution separation of open-circular and super-coiled pDNA species. The blank run verifies that no carry-over effects can be observed. This efficient separation results in increased productivity of a highly pure product.
Additional Resources
MacroSep IEX Q Brochure
Care & Use Manual
Designed and developed by
